NovaBioAssays provides the LC-MS or LC-MS/MS based analytical characterization services for Antibody-Drug Conjugates (ADCs) and other therapeutic proteins, such as bispecific antibodies, Fc fusion proteins, antibody fragments (Fab, Fc, and Nanobodies), etc. We provide a suite of biophysical, chromatography and mass spectrometry methods to characterize aggregation, charge variants, purity, sequence, clipping/PTM and glycosylation for the analyte protein. Selected services are:
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SEC-UV, SEC-MALS, and native SEC-MS
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IEX-UV, native IEX-MS, and iCIEF-UV for charge variant
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HIC-UV for hydrophobic variant
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Intact Molecular Weight Determination
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Peptide Mapping
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Post Translational Modification(PTM) Analysis
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Disulfide Bridge Mapping
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Glycosylation Analysis and Glycosylation Site Mapping/Occupancy Determination
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Protein Identification
In addition to the assays characterizing regular therapeutic proteins, we provide following services for ADC:
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Drug-to-Antibody Ratio (DAR) Determination (example shown in figure below)
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Site Occupancy
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Linkage site identification
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Analysis of Free Drug (Payload) and Naked Carrier Protein/Antibody
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Quantification of Drug-Conjugate Components
